Generator
RBS-fhlA-d
Part:BBa_K778004:Design
Designed by: Kohta Toshimitsu & Takahiro Yamada Group: iGEM12_UT-Tokyo (2012-09-23)
RBS-fhlA-d.term
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2115
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1394
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Original fhlA gene we cloned from E. coli JM109 has recognition site of PstⅠ.This illegal (inappropriate for biobrick) site was deleted by overlap extension PCR generating mutation (G to A).
Only one mutation (A to G, Thr to Ala) was found at 1906bp in this biobrick part(different from E. coli K-12 MG1655). This may have originated from cloning PCR. It is also possible that this is minor mutation of genome of JM109 we used.
Source
biobrick parts