Generator
RBS-fhlA-d

Part:BBa_K778004:Design

Designed by: Kohta Toshimitsu & Takahiro Yamada   Group: iGEM12_UT-Tokyo   (2012-09-23)

RBS-fhlA-d.term


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2115
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1394
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Original fhlA gene we cloned from E. coli JM109 has recognition site of PstⅠ.This illegal (inappropriate for biobrick) site was deleted by overlap extension PCR generating mutation (G to A).


Only one mutation (A to G, Thr to Ala) was found at 1906bp in this biobrick part(different from E. coli K-12 MG1655). This may have originated from cloning PCR. It is also possible that this is minor mutation of genome of JM109 we used.

Source

biobrick parts

References